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To determine antifungal susceptibility for the isolates using agar  dilution method: A prospective cohort study

Volume No : (2017) Volume: 05 Issue : 28 Year : 2017 Page No: 190-194

Authors : M. Suganthi, V. Dillirani, P. R. Thenmozhivalli, R. Selvi, B. J. Anand

Abstract :

The aim of the study was to standardize in vitro antifungal susceptibility testing by agar dilution method to find out the minimum inhibitory concentration (MIC) of fluconazole, ketoconazole, itraconazole, terbinafine, and griseofulvin fungal isolates of skin, hair, and nail. Various samples were collected from patients with clinically diagnosed dermatophytosis. Skin scrapings, hair, and nail were collected from one hundred and seventy patients. The antifungal drugs such as fluconazole (64–0.0625 μl/ml), ketoconazole (32–0.0313 μl/ml), itraconazole (32–0.0313 μl/ml), terbinafine (32–0.0313 μl/ml), and griseofulvin (32–0.0313 μl/ml) these water-insoluble drugs were incorporated in dissolved in dimethyl sulfoxide. The MIC was determined as the lowest concentration of the antifungal drug preventing the growth of macroscopically visible colonies on drug-containing plates when there was visible growth on the drug-free control plates. The MIC range, MIC 50, and MIC 90 for the drug griseofulvin were found to be 0.06–0.1, 0.25, and 1, respectively, for ketoconazole were found to be 0.06–0.1, 0.5, and 1, respectively, for the drug fluconazole were found to be 1–32, 8, and 16, respectively, itraconazole were found to be 0.06–4, 0.5, and 1, respectively, and for terbinafine were found to be 0.03–0.12, 0.06, and 0.12, respectively. This technique was found to be reliable, cost-effective and easy to perform with consistent results. Further results concluded that the fluconazole showed a higher MIC value when compared to other antifungal drugs.

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